Coomassie Stain solution

Coomassie Stain solution Destain solution : direction:
1) Remove SDS-PAGE gel from glass and rinse once in ddH2O in a suitable container with a lid. Try not to use a container much larger or much smaller then the gel.
2) Add enough Coomassie Stain to cover the gel by 1/2 inch (~ 1.5 cm).
3) Microwave on high power for 40 seconds to 1 minute (until the Coomassie Stain boils).
4) Incubate the gel in the Coomassie stain for 5 to 10 minutes on a rocking table. If you did not microwave the Coomassie/gel, incubate for at least 1 hour.
5) Pour off the Coomassie Stain. The Coomassie Stain can be recycled a couple of times by filtering it.
6) Rinse twice in ddH2O or used Destain solution to remove Coomassie Stain from the container.
7) Add fresh Destain solution to cover the gel by 3/4 inch (~ 2 cm).
8) Tie Kimwipes in a simple knot and place 4 of them in the Destain solution around the gel. Try to avoid laying the Kimwipes on the gel as this will cause an uneven destaining.
9) Microwave on high power for 40 seconds to 1 minute (until the Destain boils).
10) Incubate the gel in the Destain solution for 10 minutes on a rocking table. If you did not microwave the Coomassie/gel, incubate for at least 1 hour.
11) Discard the stained Kimwipes and replace with fresh knotted Kimwipes.
12) Incubate a second time for 10 minutes to overnight on a rocking table. Stop whenever the level of destaining is sufficient for you. Microwave again to speed up the process.
13) The used Destain solution can be recycled a couple of times by storing it in a sealed .

From:http://www.genetic-tests.net/readytouse-reagents/63/

GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exit the circulation and migrate into tissue, whereupon they mature into macrophages. Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. The active form of the protein is found extracellularly as a homodimer.
GM-CSF is required for the growth and development of marrow progenitor cells. It also stimulates proliferation and differentiation of granulocytes and macrophages. GM-CSF has a stimulative effect on mature macrophages, neutrophilic granulocytes and acidophilic granulocytes.

From:http://www.genetic-tests.net/Recombinant-Proteins/29/