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2011年6月7日星期二

The human elastin ELISA kit

The human elastin ELISA kit is a test solidoid sandwich enzyme-linked immunosorbent assay (ELISA). A known concentration of the standard, the unknown concentration of the sample by adding micro-ELISA plate for testing. First, incubated, and antibodies labeled with biotin at the same time. After washing, adding the HRP-labeled avidin. After further incubation and washing to remove uncombined enzyme list, then add the substrate A, B, and the enzyme acted simultaneously combo. Produce color. The depth of color and concentration of samples was proportional relationship.

Performance Kit: 1. Sensitivity: the minimum concentration of detection is lower than the standard No. 1. Linear dilution. The correlation coefficient R of the linear regression of the sample and the expected concentration is 0.990.
2.Specific: no reaction with other cytokines.
3. Repeatability coefficient of variation within the plate, inter-plate was less than 10%.

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