Human myosin heavy chain ELISA

Human myosin heavy chain ELISA is a sandwich enzyme-linked immunosorbent assay solidoid assay (ELISA). A known concentration of standard, unknown concentration of the sample by the addition of micro-ELISA plate for testing. First incubated, and biotin-labeled antibodies at the same time. After washing, addition of avidin-HRP-labeled. Remove after a further incubation and washing unbound enzyme combo, and then adding substrate A, B and enzyme combo traded simultaneously. Produce color. The color depth and concentration of samples was proportional relationship.

Kit Performance: 1 Sensitivity: the minimum detection concentration is less than 1 standard. Linear dilution. The correlation coefficient R-value of the linear regression of the sample and the expected concentration is 0.990.
2.Specific: no reaction with other cytokines.
3. Repeatability coefficient of variation of within-plate was, inter-plate less than 10%.